Incentive Sensitization - Breakpoint

Tags: Behavior · Motivation · Progressive ratio

Project: p50_paul_meyer_2020

4 loci · 4 genes with independent associations · 4 total associated genes

Significant Loci

#	Chr	Start pos	End pos	# assoc genes	# joint models	Best TWAS P	Best GWAS P	Cond GWAS P	Joint genes
1	chr1	79485178	80785451	1	1	1.55e-07	1.46e-02	3.97e-03	Zfp575
2	chr1	166093700	167491708	1	1	3.04e-09	3.70e-03	1.17e-08	Tead1
3	chr9	75969545	77365260	1	1	1.37e-68	4.35e-01	1.00e+00	Zfand2b
4	chr19	32676171	34074113	1	1	2.35e-08	1.46e-01	1.89e-02	Zdhhc1

Pleiotropic Associations

Trait	Chi² ratio	# genes⁺	# genes⁺⁺	% genes⁺⁺	Corr	Corr P	Genes
BMI with tail	5.39	2	1	25	0	1.00e+00	Tead1 Zfand2b
BMI without tail	53.65	1	1	25	0	1.00e+00	Zfand2b
Epididymis fat weight	57.61	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Heart weight	71.7	1	1	25	0	1.00e+00	Zfand2b
Left kidney weight	14.05	3	2	50	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Right kidney weight	33.45	2	2	50	0	1.00e+00	Zdhhc1 Zfand2b
Tail length	64.02	2	1	25	0	1.00e+00	Zfand2b Zfp575
Length with tail	4.78	1	0	0	0	1.00e+00	Zfand2b
Length without tail	40.87	2	1	25	0	1.00e+00	Zfand2b Zfp575
Liver weight, right	17.57	1	1	25	0	1.00e+00	Zfand2b
Parametrial fat weight	9.74	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Retroperitoneal fat weight	5.11	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Intraocular pressure	51.69	1	1	25	0	1.00e+00	Zfand2b
Number of licking bursts	32.5	1	1	25	0	1.00e+00	Zfand2b
Food consumed during 24 hour testing period	15.78	1	0	0	0	1.00e+00	Zfand2b
Times rat made contact with spout	9.25	1	0	0	0	1.00e+00	Zfand2b
Mean time between licks in bursts	9.22	1	0	0	0	1.00e+00	Zfand2b
Mean num. licks in bursts	18.13	1	0	0	0	1.00e+00	Zfand2b
Std. dev. time between licks in bursts	12.12	1	0	0	0	1.00e+00	Zfand2b
Water consumed over 24 hours	34.33	1	1	25	0	1.00e+00	Zfand2b
Patch foraging indifference point 0 sec	11.19	2	0	0	0	1.00e+00	Tead1 Zfand2b
Patch foraging indifference point AUC	16.01	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Indifference point function ln k	54.42	1	1	25	0	1.00e+00	Zfand2b
Indifference point function log k	54.42	1	1	25	0	1.00e+00	Zfand2b
Patch foraging total patch changes 0 sec	16.31	2	1	25	0	1.00e+00	Tead1 Zfand2b
Patch foraging total patch changes 12 sec	9.53	1	0	0	0	1.00e+00	Tead1
Patch foraging total patch changes 18 sec	48.28	1	1	25	0	1.00e+00	Zfand2b
Patch foraging total patch changes 24 sec	42.21	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Patch foraging total patch changes 6 sec	15.72	2	1	25	0	1.00e+00	Tead1 Zfand2b
Patch foraging time to switch 0 sec	19.75	2	1	25	0	1.00e+00	Tead1 Zfand2b
Patch foraging water rate 0 sec	39.47	1	1	25	0	1.00e+00	Zfand2b
Patch foraging water rate 12 sec	69.49	1	1	25	0	1.00e+00	Zfand2b
Patch foraging water rate 18 sec	7.84	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Patch foraging water rate 24 sec	10.78	2	0	0	0	1.00e+00	Tead1 Zfand2b
Patch foraging water rate 6 sec	15.51	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Locomotor activity	10.45	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Locomotor testing distance	47.2	1	1	25	0	1.00e+00	Zfand2b
Locomotor testing rearing	52.87	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Light reinforcement 1	14.51	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Light reinforcement 2	5.74	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Reaction time number correct	41.99	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Reaction time mean minus median	179.03	1	1	25	0	1.00e+00	Zfand2b
Reaction time mean minus median AUC	119.61	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Reaction time num false alarms	5.7	1	0	0	0	1.00e+00	Zfand2b
Reaction time num false alarms AUC	27.43	1	1	25	0	1.00e+00	Zfand2b
Reaction time trials correct on left	41.99	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Reaction time trials on left	39.48	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Reaction time mean	30.14	2	2	50	0	1.00e+00	Zdhhc1 Zfand2b
Reaction time mean AUC	26.7	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Median of all reaction times	22.05	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Reaction time omissions	46.56	1	1	25	0	1.00e+00	Zfand2b
Reaction time premature initiations	41.6	1	1	25	0	1.00e+00	Zfand2b
Std. dev. reaction times	40.77	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Reaction time trials completed	39.48	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Reaction time trials AUC	39.94	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Social response proportion	8.47	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Conditioned locomotion	5.5	1	0	0	0	1.00e+00	Zdhhc1
Cocaine response after cond. corrected	12.71	1	0	0	0	1.00e+00	Zfand2b
Cocaine response after cond. not corrected	10.73	1	0	0	0	1.00e+00	Zfand2b
Saline control response	8.43	1	0	0	0	1.00e+00	Zfand2b
Condit. Reinf. lever presses	5.55	1	0	0	0	1.00e+00	Zfand2b
Conditioned reinforcement - actives	152.54	1	1	25	0	1.00e+00	Zfand2b
Conditioned reinforcement - inactives	72.86	2	1	25	0	1.00e+00	Tead1 Zfand2b
Conditioned locomotion	8.46	3	0	0	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Intermittent access intake day 1-15 change	81.91	2	1	25	0	1.00e+00	Tead1 Zfand2b
Intermittent access intake escalation	117.41	1	1	25	0	1.00e+00	Zfand2b
Intermittent access intake escalation 2	7.7	1	0	0	0	1.00e+00	Tead1
Intermitt. access day 1 inactive lever presses	89.31	2	1	25	0	1.00e+00	Tead1 Zfand2b
Intermitt. access day 15 inactive lever presses	104.4	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfand2b
Intermittent access day 1 total infusions	45.93	3	2	50	0	1.00e+00	Tead1 Zdhhc1 Zfand2b
Intermittent access day 15 total infusions	18.09	4	1	25	-0.1	9.00e-01	Tead1 Zdhhc1 Zfand2b Zfp575
Intermittent access terminal intake (last 3 days)	14.65	3	1	25	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Intermittent access total infusions	24.03	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfp575
Intermittent access day 1 locomotion	18.95	2	1	25	0	1.00e+00	Tead1 Zfand2b
Intermittent access day 15 total locomotion	93.08	1	1	25	0	1.00e+00	Zfand2b
Intermittent access total locomotion	10.29	3	0	0	0	1.00e+00	Tead1 Zfand2b Zfp575
Intermittent access standard deviation	155.83	3	1	25	0	1.00e+00	Tead1 Zfand2b Zfp575
Cocaine induced anxiety	125.35	1	1	25	0	1.00e+00	Zfand2b
Post-drug Anxiety	8.64	1	0	0	0	1.00e+00	Zdhhc1
Baseline Anxiety	393.92	1	1	25	0	1.00e+00	Zfand2b
Lifetime Intake	22.26	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfp575
Incentive Sensitization - Responses	99.6	4	3	75	1	1.18e-04	Tead1 Zdhhc1 Zfand2b Zfp575
Progressive ratio test 1 active lever presses	21.26	3	2	50	0	1.00e+00	Tead1 Zdhhc1 Zfand2b
Progressive ratio test 1 breakpoint	21.61	2	0	0	0	1.00e+00	Tead1 Zfand2b
Progressive ratio test 1 inactive lever presses	6.43	1	0	0	0	1.00e+00	Tead1
Progressive ratio test 2 active lever presses	47.13	4	3	75	0.97	3.39e-02	Tead1 Zdhhc1 Zfand2b Zfp575
Progressive ratio test 2 breakpoint	45.29	4	2	50	0.97	3.05e-02	Tead1 Zdhhc1 Zfand2b Zfp575
Progressive ratio test 2 inactive lever presses	25.39	3	1	25	0	1.00e+00	Tead1 Zfand2b Zfp575
Total sessions with >9 infusions	27.72	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfp575
Short access day 1 total inactive lever presses	24.33	2	1	25	0	1.00e+00	Zfand2b Zfp575
Short access day 10 total inactive lever presses	21.49	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfand2b
Short access day 1 total infusions	15.66	2	0	0	0	1.00e+00	Tead1 Zdhhc1
Short access day 10 total infusions	55.42	2	1	25	0	1.00e+00	Tead1 Zdhhc1
Short access total infusions	31.58	2	1	25	0	1.00e+00	Tead1 Zdhhc1
Short access day 1 locomotion	69.81	2	1	25	0	1.00e+00	Tead1 Zfand2b
Short access day 10 total locomotion	432.47	1	1	25	0	1.00e+00	Zfand2b
Short access total locomotion	118.79	2	2	50	0	1.00e+00	Tead1 Zfand2b
Compulsive drug intake	34.92	3	1	25	0	1.00e+00	Tead1 Zfand2b Zfp575
One hour access (0.3 mA shock)	34.51	2	1	25	0	1.00e+00	Zfand2b Zfp575
One hour access (shock baseline)	20.32	2	1	25	0	1.00e+00	Zdhhc1 Zfp575
Number of responses in last shaping day	27.4	4	1	25	-0.56	4.40e-01	Tead1 Zdhhc1 Zfand2b Zfp575
Bone surface	3.9	1	0	0	0	1.00e+00	Zdhhc1
Bone volume	4.11	1	0	0	0	1.00e+00	Zdhhc1
Bone: cortical porosity	7.17	1	0	0	0	1.00e+00	Tead1
Bone: elastic displacement	5.56	1	0	0	0	1.00e+00	Zfand2b
Breakpoint value in progressive ratio session	11.08	1	0	0	0	1.00e+00	Tead1
Distance traveled before self-admin	25.03	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfp575
Distance traveled after self-admin	9.92	3	0	0	0	1.00e+00	Tead1 Zfand2b Zfp575
Delta time in open arm before/after self-admin	19.84	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Extinction: sum of active levers before priming	17.14	1	0	0	0	1.00e+00	Zfp575
Active lever presses in extinction session 6	15.11	3	0	0	0	1.00e+00	Zdhhc1 Zfand2b Zfp575
Delta ambulatory time before/after self-admin	11.38	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Time in closed arm before self-admin	22.87	3	1	25	0	1.00e+00	Tead1 Zdhhc1 Zfand2b
Time in closed arm after self-admin	7.8	2	0	0	0	1.00e+00	Tead1 Zdhhc1
Time in open arm before self-admin	22.88	2	0	0	0	1.00e+00	Zfand2b Zfp575
Sum of active levers in priming session hrs 5-6	6.97	1	0	0	0	1.00e+00	Zfand2b
Time to tail flick, vehicle, before self-admin	18.85	1	0	0	0	1.00e+00	Zdhhc1
Time to tail flick, vehicle, after self-admin	42.31	2	1	25	0	1.00e+00	Zfand2b Zfp575
Time to tail flick, test, before self-admin	10.6	2	0	0	0	1.00e+00	Zfand2b Zfp575
Delta time to tail flick, vehicle, before/after SA	74.6	1	1	25	0	1.00e+00	Zfand2b
Ambulatory time before self-admin	19.46	4	1	25	-0.91	9.24e-02	Tead1 Zdhhc1 Zfand2b Zfp575
Ambulatory time after self-admin	12.75	2	0	0	0	1.00e+00	Tead1 Zfp575
Total heroin consumption	6.81	1	0	0	0	1.00e+00	Tead1
Area under the delay curve	31.15	1	1	25	0	1.00e+00	Zfand2b
Delay disc. indifference point, 0s delay	5.71	1	0	0	0	1.00e+00	Zfand2b
Delay disc. indifference point, 16s delay	69.03	1	1	25	0	1.00e+00	Zfand2b
Delay disc. indifference point, 2s delay	7.52	1	0	0	0	1.00e+00	Zdhhc1
Delay disc. indifference point, 24s delay	27.43	1	0	0	0	1.00e+00	Zfand2b
Delay disc. indifference point, 8s delay	74.24	1	1	25	0	1.00e+00	Zfand2b
Delay discount exponential model param	16.14	1	0	0	0	1.00e+00	Zfand2b
Delay discount hyperbolic model param	15.89	1	0	0	0	1.00e+00	Zfand2b
Fecal boli incidents, locomotor time 1	176.47	1	1	25	0	1.00e+00	Zfand2b
Time >=10cm from walls, locomotor time 1	47.52	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Bouts of movement, locomotor time 1	6.19	1	0	0	0	1.00e+00	Zfand2b
Rest time, locomotor task time 1	8.65	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Vertical activity count, locomotor time 1	55.48	2	1	25	0	1.00e+00	Zfand2b Zfp575
Bouts of movement, locomotor time 2	23.9	1	0	0	0	1.00e+00	Zfand2b
Total resting periods, locomotor time 2	36.28	1	1	25	0	1.00e+00	Zfand2b
Rest time, locomotor task time 2	30.26	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Distance moved, locomotor task time 2	50.91	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Vertical activity count, locomotor time 2	12.25	2	0	0	0	1.00e+00	Zdhhc1 Zfand2b
Weight adjusted by age	12.85	2	0	0	0	1.00e+00	Zfand2b Zfp575
Seeking ratio, delayed vs. immediate footshock	9.81	2	0	0	0	1.00e+00	Zfand2b Zfp575
Locomotion in novel chamber	6.51	1	0	0	0	1.00e+00	Zfp575
Locomotion in novel chamber post-restriction	14.04	1	0	0	0	1.00e+00	Zfand2b
Progressive ratio	22.47	1	0	0	0	1.00e+00	Zdhhc1
Food seeking constrained by brief footshock	13.01	2	0	0	0	1.00e+00	Zfand2b Zfp575
Seeking ratio, punishment vs. effort	9.83	1	0	0	0	1.00e+00	Zdhhc1
Run reversals in cocaine runway, females	36.45	1	1	25	0	1.00e+00	Zfand2b
Latency to leave start box in cocaine runway	34.23	1	1	25	0	1.00e+00	Zfand2b
Latency to leave start box in cocaine runway, F	20.83	2	1	25	0	1.00e+00	Zdhhc1 Zfand2b
Latency to leave start box in cocaine runway, M	28.39	2	1	25	0	1.00e+00	Zdhhc1 Zfp575

Associations by panel

Tissue	RNA modality	# hits	Avg chisq
Adipose	alternative polyA	0	0.97
Adipose	alternative TSS	0	1
Adipose	gene expression	0	0.99
Adipose	isoform ratio	0	1.01
Adipose	intron excision ratio	0	1.01
Adipose	mRNA stability	0	1.02
BLA	alternative polyA	0	1.07
BLA	alternative TSS	0	1.04
BLA	gene expression	0	1.03
BLA	isoform ratio	0	1.04
BLA	intron excision ratio	0	0.99
BLA	mRNA stability	0	0.99
Brain	alternative polyA	0	1.09
Brain	alternative TSS	0	1.02
Brain	gene expression	0	1.01
Brain	isoform ratio	0	1.05
Brain	intron excision ratio	0	0.98
Brain	mRNA stability	0	1.02
Eye	alternative polyA	0	1.16
Eye	alternative TSS	0	1.2
Eye	gene expression	0	1.08
Eye	isoform ratio	0	0.98
Eye	intron excision ratio	0	0.98
Eye	mRNA stability	0	1.01
IL	alternative polyA	0	0.98
IL	alternative TSS	0	1.1
IL	gene expression	0	1.04
IL	isoform ratio	0	0.99
IL	intron excision ratio	0	1.02
IL	mRNA stability	0	1.05
LHb	alternative polyA	0	1.01
LHb	alternative TSS	0	1.13
LHb	gene expression	0	1
LHb	isoform ratio	0	1.08
LHb	intron excision ratio	0	1.03
LHb	mRNA stability	0	1.04
Liver	alternative polyA	0	1.03
Liver	alternative TSS	0	1.07
Liver	gene expression	0	1.03
Liver	isoform ratio	0	1.02
Liver	intron excision ratio	0	1.03
Liver	mRNA stability	0	1.01
NAcc	alternative polyA	0	1.04
NAcc	alternative TSS	1	1.02
NAcc	gene expression	0	1.01
NAcc	isoform ratio	0	1.01
NAcc	intron excision ratio	0	1.02
NAcc	mRNA stability	1	1.05
OFC	alternative polyA	0	0.99
OFC	alternative TSS	0	1.11
OFC	gene expression	0	1
OFC	isoform ratio	0	0.99
OFC	intron excision ratio	0	0.95
OFC	mRNA stability	0	0.98
PL	alternative polyA	0	1.02
PL	alternative TSS	0	1
PL	gene expression	0	1.01
PL	isoform ratio	0	1.02
PL	intron excision ratio	1	1.04
PL	mRNA stability	1	1.04
pVTA	alternative polyA	0	1.12
pVTA	alternative TSS	0	1.08
pVTA	gene expression	0	1.03
pVTA	isoform ratio	0	1.05
pVTA	intron excision ratio	0	1.06
pVTA	mRNA stability	0	1.11
RMTg	alternative polyA	0	1
RMTg	alternative TSS	0	0.97
RMTg	gene expression	0	1.03
RMTg	isoform ratio	0	0.98
RMTg	intron excision ratio	0	1.07
RMTg	mRNA stability	0	1.18