Hub : Traits

Progressive ratio

Progressive ratio, i.e. food-seeking constrained by escalating effort schedule. The last successfully completed ratio or shock intensity endured before timeout. Animals are food restricted to 85% of initial body weight, trained to lever-press for food pellets (45 mg) until stable response rates were reached, then tested either on the progressive ratio (PR) task with progressively increasing effort requirements (lever-presses required per pellet) or progressively increasing punishment (i.e., a foot-shock of increasing intensity presented 1 s after food pellet delivery, whose magnitude increases ~25% every 3 trials). In the PR task, rats time out if 2 min passed without a lever press. In the punishment task, a timeout is defined by three consecutive trials with >30 s passing without a lever press. For both tasks, the break point is the last successfully completed ratio or shock intensity endured before timeout.

Tags: Behavior · Motivation

Project: u01_tom_jhou

1 significantly associated model · 1 unique gene

Significant Loci

# chr p0 p1 # assoc genes # joint models best TWAS P best SNP P cond SNP P % var exp joint genes
1 10 62271668 63667097 1 1 1.3e-08 0.54 4.5e-09 -8850 Eral1

p0: physical position of the start of the locus. p1: physical position of the end of the locus. # joint models: number of models selected by step-wise conditional analysis.

Pleiotropic Associations

Trait chisq ratio # genes+ # genes++ % genes++ corr corr P genes
Heart weight 3.6 1 0 0 0 1 Eral1
Left kidney weight 1.5 1 0 0 0 1 Eral1
Food consumed during 24 hour testing period 14.7 1 0 0 0 1 Eral1
Times rat made contact with spout 19.6 1 0 0 0 1 Eral1
Mean time between licks in bursts 5.6 1 0 0 0 1 Eral1
Mean num. licks in bursts 16.6 1 0 0 0 1 Eral1
Std. dev. time between licks in bursts 13.9 1 0 0 0 1 Eral1
Intermittent access intake escalation 2 6.5 1 0 0 0 1 Eral1
Intermitt. access day 1 inactive lever presses 5.8 1 0 0 0 1 Eral1
Intermitt. access day 15 inactive lever presses 19.8 1 0 0 0 1 Eral1
Intermittent access total locomotion 7.3 1 0 0 0 1 Eral1
Short access day 10 total inactive lever presses 4.2 1 0 0 0 1 Eral1
Short access day 1 total infusions 28.5 1 1 100 0 1 Eral1
Short access total infusions 17.6 1 0 0 0 1 Eral1
Short access day 1 locomotion 23.2 1 0 0 0 1 Eral1
Short access total locomotion 23.8 1 0 0 0 1 Eral1
Breakpoint value in progressive ratio session 7.3 1 0 0 0 1 Eral1
Distance traveled before self-admin 58.4 1 1 100 0 1 Eral1
Distance traveled after self-admin 7.5 1 0 0 0 1 Eral1
Delta time in open arm before/after self-admin 8.0 1 0 0 0 1 Eral1
Extinction: sum of active levers before priming 8.2 1 0 0 0 1 Eral1
Active lever presses in extinction session 6 20.2 1 0 0 0 1 Eral1
Delta distance traveled before/after self-admin 8.5 1 0 0 0 1 Eral1
Delta ambulatory time before/after self-admin 37.5 1 1 100 0 1 Eral1
Time in open arm after self-admin 10.5 1 0 0 0 1 Eral1
Time to tail flick, vehicle, before self-admin 10.1 1 0 0 0 1 Eral1
Time to tail flick, vehicle, after self-admin 4.9 1 0 0 0 1 Eral1
Ambulatory time before self-admin 49.1 1 1 100 0 1 Eral1
Ambulatory time after self-admin 6.6 1 0 0 0 1 Eral1
Total heroin consumption 3.3 1 0 0 0 1 Eral1
Area under the delay curve 45.5 1 1 100 0 1 Eral1
Delay disc. indifference point, 16s delay 63.9 1 1 100 0 1 Eral1
Delay disc. indifference point, 24s delay 9.5 1 0 0 0 1 Eral1
Delay disc. indifference point, 4s delay 6.5 1 0 0 0 1 Eral1
Delay disc. indifference point, 8s delay 71.9 1 1 100 0 1 Eral1
Delay discount exponential model param 36.3 1 1 100 0 1 Eral1
Delay discount hyperbolic model param 29.7 1 1 100 0 1 Eral1
Rest time, locomotor task time 1 15.2 1 0 0 0 1 Eral1
Distance moved, locomotor task time 1 3.7 1 0 0 0 1 Eral1
Time >=10cm from walls, locomotor time 2 39.8 1 1 100 0 1 Eral1
Rest time, locomotor task time 2 5.6 1 0 0 0 1 Eral1
Distance moved, locomotor task time 2 13.4 1 0 0 0 1 Eral1
Vertical activity count, locomotor time 2 8.7 1 0 0 0 1 Eral1
Weight adjusted by age 3.4 1 0 0 0 1 Eral1
Locomotion in novel chamber post-restriction 13.5 1 0 0 0 1 Eral1
Food seeking constrained by brief footshock 14.0 1 0 0 0 1 Eral1
Seeking ratio, punishment vs. effort 10.2 1 0 0 0 1 Eral1
Run reversals in cocaine runway, males 48.1 1 1 100 0 1 Eral1
Latency to leave start box in cocaine runway 12.6 1 0 0 0 1 Eral1
Latency to leave start box in cocaine runway, M 38.5 1 1 100 0 1 Eral1

chisq ratio: Average Chi2 statistic for the selected genes in the secondary trait, divided by the average statistic for all genes in the secondary trait. # genes+: Shared significant genes at Bonferroni correction. # genes++: Shared significant genes at transcriptome-wide significance. % genes++: Number of [++] genes as a percentage of primary trait’s total joint genes. corr: Correlation of effect sizes across the [+] genes.

Associations by panel

tissue modality # hits % hits/tests avg chisq
Adipose alternative polyA 0 0.000 1.24
Adipose alternative TSS 0 0.000 1.10
Adipose gene expression 1 0.012 1.11
Adipose isoform ratio 0 0.000 1.17
Adipose intron excision ratio 0 0.000 1.19
Adipose mRNA stability 0 0.000 1.14
BLA alternative polyA 0 0.000 1.25
BLA alternative TSS 0 0.000 1.12
BLA gene expression 0 0.000 1.18
BLA isoform ratio 0 0.000 1.14
BLA intron excision ratio 0 0.000 1.21
BLA mRNA stability 0 0.000 1.10
Brain alternative polyA 0 0.000 1.16
Brain alternative TSS 0 0.000 1.27
Brain gene expression 0 0.000 1.14
Brain isoform ratio 0 0.000 1.15
Brain intron excision ratio 0 0.000 1.19
Brain mRNA stability 0 0.000 1.10
Eye alternative polyA 0 0.000 0.86
Eye alternative TSS 0 0.000 0.99
Eye gene expression 0 0.000 1.24
Eye isoform ratio 0 0.000 1.15
Eye intron excision ratio 0 0.000 1.19
Eye mRNA stability 0 0.000 0.93
IL alternative polyA 0 0.000 1.16
IL alternative TSS 0 0.000 1.28
IL gene expression 0 0.000 1.19
IL isoform ratio 0 0.000 1.18
IL intron excision ratio 0 0.000 1.24
IL mRNA stability 0 0.000 1.11
LHb alternative polyA 0 0.000 1.39
LHb alternative TSS 0 0.000 1.11
LHb gene expression 0 0.000 1.21
LHb isoform ratio 0 0.000 1.24
LHb intron excision ratio 0 0.000 1.38
LHb mRNA stability 0 0.000 1.10
Liver alternative polyA 0 0.000 1.33
Liver alternative TSS 0 0.000 1.18
Liver gene expression 0 0.000 1.14
Liver isoform ratio 0 0.000 1.24
Liver intron excision ratio 0 0.000 1.15
Liver mRNA stability 0 0.000 1.09
NAcc alternative polyA 0 0.000 1.19
NAcc alternative TSS 0 0.000 1.13
NAcc gene expression 0 0.000 1.14
NAcc isoform ratio 0 0.000 1.11
NAcc intron excision ratio 0 0.000 1.19
NAcc mRNA stability 0 0.000 1.12
OFC alternative polyA 0 0.000 1.06
OFC alternative TSS 0 0.000 1.43
OFC gene expression 0 0.000 1.18
OFC isoform ratio 0 0.000 1.14
OFC intron excision ratio 0 0.000 1.37
OFC mRNA stability 0 0.000 1.13
PL alternative polyA 0 0.000 1.17
PL alternative TSS 0 0.000 1.06
PL gene expression 0 0.000 1.16
PL isoform ratio 0 0.000 1.13
PL intron excision ratio 0 0.000 1.26
PL mRNA stability 0 0.000 1.06

avg chisq: Average Chi2 (squared Z-score) across all models in this panel for this trait.