Hub : Genes

Tcam1

Models

All models for this gene with cis-heritability P-value <0.01 were tested and are shown here. In each tissue, a gene can have zero, one, or multiple RNA phenotypes for each RNA modality. After the heritability statistics, subsequent columns show cross-validation performance (R2 and P-value) of each modeling method. The best-performing method for each models is used for TWAS.

# Tissue Modality RNA phenotype h2 h2 SE h2 P xQTL R2 BLUP R2 ENET R2 LASSO R2 xQTL P BLUP P ENET P LASSO P
1 BLA gene expression ENSRNOG00000010929 0.56 0.12 0.0e+00 0.44 0.48 0.5 0.5 8.3e-26 1.7e-28 1.5e-30 1.4e-30
2 Brain gene expression ENSRNOG00000010929 0.25 0.08 0.0e+00 0.21 0.26 0.27 0.26 2.4e-19 5.2e-24 6.1e-25 7.3e-24
3 IL gene expression ENSRNOG00000010929 0.44 0.12 7.6e-08 0.18 0.29 0.25 0.23 3.3e-05 1.0e-07 1.0e-06 3.6e-06
4 OFC gene expression ENSRNOG00000010929 0.15 0.09 5.8e-03 0.09 0.07 0.02 0.06 4.2e-03 1.1e-02 8.7e-02 2.0e-02
5 PL gene expression ENSRNOG00000010929 0.36 0.11 0.0e+00 0.35 0.35 0.36 0.36 2.9e-27 3.3e-27 7.6e-29 1.5e-28

Trait associations

The numbered columns correspond to the numbered models in the table above. They contain the TWAS Z-score for each trait-model pair. Colors indicate strong Z-scores (below -2 or above 2): lower negative Z-scores are darker blue, higher positive Z-scores are darker red.

Trait Avg chi2 ratio Avg chi2 Max chi2 1 2 3 4 5
BMI with tail0.72.48.41.0-0.02.90.51.6
BMI without tail3.26.615.02.51.43.91.82.6
Body weight2.39.518.12.71.94.32.93.2
Epididymis fat weight1.43.24.21.81.52.01.62.0
Glucose0.71.01.9-1.0-1.1-0.6-1.4-0.9
Heart weight0.20.20.90.2-0.00.9-0.20.5
Left kidney weight0.72.15.0-1.5-2.2-0.2-1.5-1.0
Right kidney weight0.71.94.4-1.4-2.1-0.6-1.2-1.1
Tail length1.75.26.12.32.12.32.52.2
Length with tail0.93.46.11.61.71.52.51.7
Length without tail0.10.20.4-0.40.0-0.70.6-0.1
Liver weight, left0.30.50.80.80.90.10.90.5
Liver weight, right1.11.93.3-1.4-1.8-1.3-1.1-1.2
Parametrial fat weight0.10.30.90.50.2-0.11.00.4
Retroperitoneal fat weight1.13.84.61.91.62.12.02.2
Intraocular pressure2.12.96.21.51.02.51.02.0
Extensor digitorum longus weight0.20.61.20.70.61.1-0.70.7
Soleus weight0.10.30.90.3-0.01.00.60.4
Tibialis anterior weight1.03.55.21.91.62.31.52.0
Tibia length0.41.23.0-1.3-1.7-0.5-0.6-0.7
Number of licking bursts0.00.10.1-0.2-0.2-0.2-0.3-0.2
Food consumed during 24 hour testing period4.45.37.12.42.12.71.92.4
Times rat made contact with spout0.40.40.60.60.40.70.80.6
Mean time between licks in bursts0.20.30.80.40.20.90.30.7
Mean num. licks in bursts0.50.60.80.80.60.90.90.8
Std. dev. time between licks in bursts0.10.10.20.40.40.50.30.3
Water consumed over 24 hours0.00.00.1-0.0-0.20.2-0.00.1
Indifference point 0 sec4.77.69.12.72.52.82.83.0
Indifference point AUC1.82.12.41.31.61.51.51.5
Indifference point function ln k0.50.71.3-0.5-1.1-0.8-0.9-0.9
Indifference point function log k0.50.71.3-0.5-1.1-0.8-0.9-0.9
Delay discounting total patch changes 0 sec4.87.89.62.72.52.92.83.1
Delay discounting total patch changes 12 sec7.09.312.23.03.52.72.93.2
Delay discounting total patch changes 18 sec3.74.86.21.92.12.52.02.4
Delay discounting total patch changes 24 sec3.44.35.31.82.02.02.22.3
Delay discounting total patch changes 6 sec2.23.44.01.71.61.92.02.0
Delay discounting time to switch 0 sec0.30.50.8-0.7-0.4-0.9-0.7-0.8
Delay discounting water rate 0 sec1.42.33.51.40.81.91.61.7
Delay discounting water rate 12 sec2.03.06.2-1.8-2.5-1.0-1.5-1.5
Delay discounting water rate 18 sec0.81.02.6-0.8-1.6-0.9-0.4-0.8
Delay discounting water rate 24 sec0.20.31.0-0.3-1.0-0.3-0.5-0.3
Delay discounting water rate 6 sec0.50.72.0-0.7-1.4-0.6-0.5-0.7
Locomotor activity0.50.92.20.90.21.50.70.9
Locomotor testing distance2.13.56.61.81.22.61.61.9
Locomotor testing rearing1.82.84.81.71.52.21.41.6
Light reinforcement 10.91.72.21.51.51.10.91.5
Light reinforcement 20.71.01.41.21.10.51.00.9
Reaction time number correct0.71.11.8-0.8-1.4-0.6-1.3-0.8
Reaction time mean minus median0.20.30.5-0.7-0.3-0.7-0.4-0.6
Reaction time mean minus median AUC1.21.51.7-1.3-1.0-1.3-1.2-1.3
Reaction time num false alarms2.23.14.11.71.72.01.81.5
Reaction time num false alarms AUC2.33.24.01.81.71.92.01.5
Reaction time trials correct on left0.71.11.8-0.8-1.4-0.6-1.3-0.8
Reaction time trials on left0.81.11.9-0.8-1.4-0.6-1.3-0.8
Reaction time mean1.21.73.0-1.4-0.8-1.7-1.0-1.4
Reaction time mean AUC1.41.93.3-1.4-0.8-1.8-1.2-1.5
Median of all reaction times1.31.73.1-1.4-0.8-1.8-1.1-1.3
Reaction time omissions0.20.30.6-0.6-0.2-0.8-0.2-0.5
Reaction time false alarm rate2.53.65.01.72.01.82.21.6
Reaction time premature initiation rate1.21.62.31.20.81.51.31.4
Reaction time premature initiations0.50.71.30.90.21.10.60.9
Std. dev. reaction times0.60.91.8-1.0-0.4-1.3-0.6-1.0
Reaction time trials completed0.81.11.9-0.8-1.4-0.6-1.3-0.8
Reaction time trials AUC0.71.01.8-0.8-1.3-0.6-1.3-0.8
Social response proportion0.20.20.6-0.2-0.60.4-0.8-0.1
Social responses0.20.20.70.40.10.9-0.00.3
Social time0.10.10.30.2-0.20.5-0.10.4
Conditioned locomotion1.41.72.41.31.51.11.11.5
Cocaine response after cond. corrected0.80.81.6-1.0-1.3-0.7-0.9-0.6
Cocaine response after cond. not corrected2.42.74.0-1.7-2.0-1.6-1.3-1.4
Cocaine response before conditioning0.91.11.6-1.0-1.0-1.3-0.6-1.2
Saline control response3.54.65.22.12.22.32.02.2
Condit. Reinf. active minus inactive responses2.53.15.11.81.51.62.31.6
Condit. Reinf. active-inactive response ratio1.01.02.31.10.80.61.50.7
Condit. Reinf. active responses4.76.58.32.52.42.42.92.6
Condit. Reinf. inactive responses3.64.45.51.92.22.21.82.4
Condit. Reinf. lever presses1.11.51.91.11.01.41.41.1
Condit. Reinf. lever reinforcers received3.85.37.12.22.12.22.72.3
Pavlov. Cond. lever latency0.10.20.3-0.4-0.4-0.5-0.4-0.4
Pavlov. Cond. magazine entry latency2.42.73.7-1.7-1.5-1.6-1.9-1.6
Pavlov. Cond. change in total contacts0.40.61.00.81.00.70.70.7
Pavlov. Cond. index score0.10.10.2-0.4-0.3-0.1-0.4-0.3
Pavlov. Cond. latency score0.20.30.4-0.6-0.6-0.3-0.6-0.5
Pavlov. Cond. lever contacts0.10.20.30.40.40.50.40.4
Pavlov. Cond. magazine entry number3.34.14.92.12.01.92.21.9
Pavlov. Cond. intertrial magazine entries13.918.021.54.24.03.94.64.4
Pavlov. Cond. lever-magazine prob. diff.0.20.20.3-0.5-0.4-0.3-0.5-0.4
Pavlov. Cond. response bias0.00.00.0-0.1-0.10.0-0.2-0.0
Conditioned reinforcement - actives1.11.01.81.00.81.31.01.0
Conditioned reinforcement - inactives0.91.01.60.90.71.31.10.9
Conditioned locomotion2.02.43.41.41.21.71.81.4
Intermittent access intake day 1-15 change0.20.20.20.40.50.50.50.4
Intermittent access intake escalation0.00.00.00.10.20.10.10.0
Intermittent access intake escalation 20.30.30.50.50.70.40.70.5
Intermitt. access day 1 inactive lever presses2.62.84.91.51.41.62.21.5
Intermitt. access day 15 inactive lever presses1.81.92.81.41.11.31.71.5
Intermitt. access escalation Index3.12.83.5-1.5-1.6-1.6-1.9-1.7
Intermittent access day 1 total infusions0.91.02.01.00.51.40.81.0
Intermittent access day 15 total infusions0.91.02.01.00.61.40.81.0
Intermittent access terminal intake (last 3 days)2.72.93.71.81.41.91.71.7
Intermittent access total infusions2.93.24.51.81.42.11.81.8
Intermittent access day 1 locomotion2.92.95.01.70.92.21.91.6
Intermittent access day 15 total locomotion4.24.45.82.01.72.42.42.0
Intermittent access total locomotion6.27.09.42.52.03.13.02.5
Intermittent access standard deviation1.11.21.71.00.71.31.31.0
Cocaine induced anxiety0.70.81.8-0.9-1.3-0.6-0.6-0.8
Post-drug Anxiety1.01.12.3-1.1-1.5-0.8-0.8-0.9
Baseline Anxiety0.80.91.0-1.0-0.9-0.9-1.0-0.9
Lifetime Intake3.64.05.42.01.62.32.02.0
Incentive Sensitization - Responses2.82.83.91.71.31.62.01.7
Incentive Sensitization - Breakpoint2.32.43.51.51.21.41.91.6
Progressive ratio test 1 active lever presses4.24.35.42.01.72.32.22.0
Progressive ratio test 1 breakpoint6.46.68.22.62.22.92.62.5
Progressive ratio test 1 inactive lever presses5.15.46.72.42.02.62.42.2
Progressive ratio test 2 active lever presses4.44.76.22.11.72.32.52.2
Progressive ratio test 2 breakpoint4.95.57.02.31.82.52.62.3
Progressive ratio test 2 inactive lever presses4.95.76.42.42.02.52.52.5
Total sessions with >9 infusions0.50.61.30.70.31.20.50.8
Short access day 1 total inactive lever presses0.30.30.70.4-0.00.80.70.4
Short access day 10 total inactive lever presses1.31.41.81.31.21.11.01.2
Short access day 1 total infusions2.02.13.81.51.11.91.11.4
Short access day 10 total infusions6.77.49.42.82.43.12.72.7
Short access total infusions3.94.36.32.21.72.51.91.9
Short access day 1 locomotion4.85.18.22.21.72.92.12.2
Short access day 10 total locomotion10.711.714.23.33.03.83.73.3
Short access total locomotion6.36.410.22.42.03.22.52.4
Compulsive drug intake2.82.84.1-1.8-2.0-1.5-1.4-1.5
One hour access (0.3 mA shock)1.21.42.01.21.11.41.11.1
One hour access (shock baseline)2.73.13.61.81.91.81.71.6
Number of responses in last shaping day0.60.61.40.60.21.20.80.6
Context. condit. distance diff. score0.20.20.50.40.30.7-0.10.4
Locomotion velocity, session 10.10.10.40.2-0.30.10.60.1
Locomotion distance, session 10.10.10.3-0.2-0.50.1-0.10.2
Locomotion velocity, session 20.10.10.20.00.4-0.20.40.1
Locomotion distance, session 20.00.00.0-0.2-0.2-0.1-0.2-0.1
Locomotion velocity, session 30.70.81.3-1.0-1.1-0.6-0.8-0.8
Locomotion distance, session 30.20.20.4-0.6-0.6-0.4-0.2-0.4
Stereotopy head waving bouts, day 30.60.71.2-0.8-0.9-1.1-0.5-0.8
Stereotopy head waving duration, day 30.10.20.3-0.4-0.3-0.5-0.4-0.4
Locomotion velocity, session 70.60.70.9-0.9-0.9-0.9-0.7-0.7
Locomotion distance, session 70.20.20.40.30.40.5-0.10.7
Stereotopy head waving bouts, day 70.91.01.4-1.1-1.0-1.2-0.8-0.9
Stereotopy head waving duration, day 72.53.15.0-1.8-1.3-2.2-1.5-1.8
Locomotion velocity, session 80.50.60.90.80.70.90.80.8
Locomotion distance, session 80.20.30.40.70.70.70.20.6
Degree of sensitization distance1.21.31.61.21.31.20.51.3
Degree of sensitization stereotypy1.61.72.3-1.5-1.2-1.3-1.3-1.2
Condit. Reinf. active minus inactive responses2.42.73.01.61.51.71.71.6
Condit. Reinf. active-inactive response ratio0.91.01.11.01.00.91.00.9
Condit. Reinf. active responses3.44.24.82.02.11.92.22.0
Condit. Reinf. inactive responses1.01.21.51.11.20.91.21.1
Incentive salience index mean1.21.42.71.11.21.01.61.0
Condit. Reinf. lever presses0.10.10.40.10.4-0.20.6-0.1
Time in familiar zone, hab. session 12.22.02.91.41.61.31.01.7
Time in novel zone, hab. session 12.42.33.3-1.5-1.8-1.2-1.2-1.7
Total zone transitions, hab. session 10.20.30.90.3-0.20.90.20.6
Total locomotion distance, hab. session 10.10.20.30.1-0.40.60.40.4
Locomotion velocity, hab. session 10.20.30.60.40.00.80.70.7
Time in familiar zone, hab. session 22.52.63.21.51.81.71.41.6
Time in novel zone, hab. session 23.13.14.2-1.6-2.1-1.8-1.6-1.7
Total zone transitions, hab. session 20.10.20.40.40.20.60.20.5
Total locomotion distance, hab. session 20.50.71.10.90.70.80.71.1
Locomotion velocity, hab. session 20.40.61.00.80.60.70.71.0
Novel to familiar place preference ratio0.10.10.30.20.10.40.50.2
Novelty place preference0.00.00.10.0-0.10.20.3-0.0
Time in novel zone, NPP test0.00.00.1-0.2-0.30.00.1-0.3
Total zone transitions, NPP test0.00.00.10.1-0.10.3-0.00.3
Total locomotion distance, NPP test0.10.10.30.30.00.30.50.4
Locomotion velocity, NPP test0.40.50.80.70.50.80.70.9
Pavlov. Cond. lever latency0.40.61.9-0.5-0.4-0.6-1.4-0.3
Pavlov. Cond. magazine entry latency1.72.24.71.41.41.12.21.1
Pavlov. Cond. change in total contacts1.51.63.51.21.20.91.90.9
Pavlov. Cond. index score1.41.84.11.21.21.02.00.9
Pavlov. Cond. latency score1.11.43.41.11.11.01.80.8
Pavlov. Cond. lever contacts0.50.61.80.60.60.61.30.4
Pavlov. Cond. magazine entry number1.21.53.8-1.2-1.2-0.7-2.0-0.7
Pavlov. Cond. intertrial magazine entries3.74.45.92.12.12.31.42.4
Pavlov. Cond. lever-magazine prob. diff.1.11.43.61.01.00.91.90.7
Pavlov. Cond. response bias0.91.13.60.90.90.51.90.6
Bone: apparent density0.20.41.0-0.1-0.3-0.81.0-0.3
Bone surface0.91.65.30.90.70.62.30.9
Bone volume0.81.44.60.90.70.52.10.8
Bone: connectivity density0.20.41.60.30.2-0.41.30.1
Bone: cortical apparent density2.73.54.4-1.8-1.8-1.9-2.1-1.7
Bone: cortical area0.40.61.50.3-0.21.11.20.6
Bone: cortical porosity1.61.84.80.80.51.52.20.9
Bone: cortical porosity0.80.92.30.4-0.01.31.50.5
Bone: cortical thickness5.18.414.6-3.2-3.8-2.1-2.5-2.6
Bone: cortical thickness5.46.38.8-2.8-3.0-2.0-2.3-2.4
Bone: cortical tissue density2.53.34.2-1.7-1.7-1.9-2.0-1.7
Bone: elastic displacement3.43.96.2-2.1-2.5-1.6-1.6-1.8
Bone: elastic work0.71.02.5-1.1-1.6-0.6-0.3-0.9
Bone: endosteal estimation10.021.528.14.64.64.45.34.2
Bone: endosteal perimeter11.623.931.24.74.64.95.64.5
Bone: final force0.10.20.5-0.4-0.70.00.4-0.3
Bone: final moment0.20.30.7-0.5-0.8-0.10.4-0.4
Bone: marrow area9.520.527.04.54.54.35.24.1
Bone: maximum diameter2.13.96.81.81.52.02.61.8
Bone: maximum force0.40.81.50.50.11.21.20.8
Bone: maximum moment0.40.61.40.40.01.11.20.7
Bone: minimum diameter9.016.623.43.93.44.24.83.9
Bone: periosteal estimation5.010.316.73.02.63.24.12.9
Bone: periosteal perimeter1.83.06.31.40.92.02.51.4
Bone: post-yield work1.41.82.71.21.11.61.41.3
Bone: stiffness0.40.60.90.60.81.00.80.7
Bone: tissue strength1.51.41.9-1.3-1.3-0.9-1.4-1.0
Bone: trabecular number0.30.41.80.30.2-0.21.30.3
Bone: trabecular spacing0.10.20.30.30.60.5-0.40.2
Bone: trabecular thickness0.40.52.00.3-0.10.11.40.5
Bone: trabecular tissue density1.41.93.6-1.5-1.9-1.0-1.4-1.0
Breakpoint value in progressive ratio session0.80.91.21.11.01.01.00.8
Distance traveled before self-admin0.80.91.2-0.8-1.1-0.5-1.1-1.0
Distance traveled after self-admin0.20.20.4-0.4-0.20.1-0.5-0.6
Delta time in closed arm before/after self-admin0.60.60.9-0.8-0.7-0.9-0.3-0.9
Delta time in open arm before/after self-admin0.50.50.80.90.90.5-0.10.7
Diff in mean of infusions in LGA sessions0.40.50.9-0.7-0.8-0.2-0.9-0.7
Extinction: sum of active levers before priming1.41.42.01.41.41.20.71.1
Active lever presses in extinction session 62.12.02.41.41.31.61.51.3
Delta distance traveled before/after self-admin0.10.10.2-0.4-0.00.0-0.2-0.5
Delta ambulatory time before/after self-admin0.10.10.20.00.50.10.30.0
Time in closed arm before self-admin1.61.92.4-1.4-1.5-1.1-1.3-1.5
Time in closed arm after self-admin2.82.93.8-1.8-1.6-1.7-1.3-2.0
Time in open arm before self-admin1.11.11.7-1.2-1.3-0.7-0.9-1.0
Time in open arm after self-admin1.31.41.81.31.11.30.61.4
Sum of active levers in priming session hrs 5-60.20.20.50.60.70.4-0.20.3
Time to tail flick, vehicle, before self-admin1.31.31.8-1.2-1.3-1.3-0.8-1.1
Time to tail flick, vehicle, after self-admin0.80.91.31.10.90.60.81.2
Time to tail flick, test, before self-admin0.60.60.7-0.8-0.6-0.8-0.8-0.7
Time to tail flick, test, after self-admin0.20.20.2-0.4-0.3-0.5-0.4-0.4
Delta time to tail flick, vehicle, before/after SA3.63.54.52.12.11.41.81.9
Delta time to tail flick, test, before/after SA0.00.00.10.20.3-0.1-0.00.0
Ambulatory time before self-admin0.40.50.8-0.6-0.9-0.3-0.8-0.7
Ambulatory time after self-admin0.00.00.1-0.2-0.00.3-0.0-0.3
Total heroin consumption0.00.00.10.20.30.10.30.1
Area under the delay curve0.00.00.10.0-0.10.20.30.3
Delay disc. indifference point, 0s delay0.30.40.8-0.6-0.3-0.9-0.6-0.5
Delay disc. indifference point, 16s delay0.40.50.70.50.20.80.80.8
Delay disc. indifference point, 2s delay0.10.10.40.20.3-0.40.60.3
Delay disc. indifference point, 24s delay2.72.63.91.41.02.01.71.8
Delay disc. indifference point, 4s delay1.51.62.1-1.5-1.4-1.2-1.0-1.2
Delay disc. indifference point, 8s delay0.20.20.3-0.4-0.5-0.6-0.3-0.2
Delay discount exponential model param0.10.10.3-0.2-0.1-0.3-0.5-0.5
Delay discount hyperbolic model param0.10.10.2-0.1-0.0-0.1-0.4-0.3
Fecal boli incidents, locomotor time 10.10.10.20.40.50.40.20.4
Change in fecal boli incidents, locomotor task0.00.00.1-0.3-0.10.1-0.30.2
Time >=10cm from walls, locomotor time 12.82.93.5-1.6-1.5-1.6-1.9-1.9
Bouts of movement, locomotor time 10.10.10.2-0.3-0.4-0.4-0.5-0.3
Total resting periods, locomotor time 10.20.30.4-0.4-0.5-0.6-0.5-0.4
Rest time, locomotor task time 10.30.31.2-0.3-0.0-1.1-0.5-0.4
Distance moved, locomotor task time 10.40.40.90.30.00.90.90.6
Vertical activity count, locomotor time 10.10.10.3-0.2-0.30.30.50.1
Time >=10cm from walls, locomotor time 20.91.01.5-0.8-1.0-1.0-0.9-1.2
Bouts of movement, locomotor time 20.00.00.10.10.30.2-0.30.1
Total resting periods, locomotor time 20.10.10.3-0.20.0-0.1-0.6-0.1
Rest time, locomotor task time 21.92.23.3-1.3-1.0-1.8-1.8-1.4
Distance moved, locomotor task time 20.10.10.4-0.1-0.30.20.6-0.1
Vertical activity count, locomotor time 21.51.63.01.20.91.31.71.1
Weight adjusted by age0.10.10.3-0.2-0.10.0-0.6-0.1
Seeking ratio, delayed vs. immediate footshock0.40.50.90.60.51.00.40.8
Locomotion in novel chamber0.70.91.8-1.2-1.3-0.6-0.4-0.9
Locomotion in novel chamber post-restriction1.01.11.71.21.10.71.30.9
Progressive ratio0.10.10.3-0.4-0.1-0.6-0.0-0.3
Food seeking constrained by brief footshock3.23.94.61.92.21.82.11.8
Seeking ratio, punishment vs. effort1.51.82.31.41.31.51.21.3
Run reversals in cocaine runway, females0.50.51.2-0.8-1.1-0.3-0.5-0.6
Run reversals in cocaine runway, males0.30.30.5-0.5-0.7-0.3-0.4-0.5
Latency to leave start box in cocaine runway0.40.40.6-0.6-0.8-0.5-0.8-0.7
Latency to leave start box in cocaine runway, F1.31.52.1-1.3-1.4-0.7-1.2-1.3
Latency to leave start box in cocaine runway, M0.10.10.20.40.3-0.30.00.2
Cd content in liver1.61.72.2-1.2-1.0-1.3-1.5-1.4
Co content in liver0.00.00.1-0.10.1-0.2-0.2-0.0
Cu content in liver0.30.40.60.60.70.20.70.7
Fe content in liver0.10.10.2-0.30.1-0.4-0.3-0.4
K content in liver0.50.51.20.61.1-0.20.90.5
Mg content in liver0.50.51.50.51.2-0.80.50.2
Mn content in liver0.40.51.8-0.5-0.0-1.3-0.2-0.7
Na content in liver0.50.51.30.71.10.10.80.3
Rb content in liver2.63.35.6-1.8-1.1-2.4-1.9-1.7
Se content in liver0.40.40.8-0.7-0.4-0.8-0.9-0.4
Sr content in liver0.10.10.3-0.2-0.60.30.20.0
Zn content in liver0.10.10.2-0.3-0.3-0.50.10.1